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A CHIKV RNAemia by RT-qPCR of plasma. Group log 10 gc/mL results with symbols representing the mean log 10 gc/mL of the NHP groups shown and error bars representing standard error of the mean. The validated CHIKV RT-qPCR assay LOD (dashed line) and LLOQ (dotted line) were 292 and 931 gc/mL, respectively. Results <LOD were imputed to half-LOD ( ~ 146 gc/mL) and values between LOD and LLOQ were imputed to half (LOD + LLOQ) (611.5 gc/mL) for calculations and plotting. B Relationship between peak viremia by RT-qPCR vs pre-challenge Day 0 SNA titers. Day 0 NT 80 and maximum gc/mL results were log 10 -transformed. Least squares linear regression was performed with the solid line and shading representing the regression result and 95% confidence interval, respectively. Each symbol represents an individual animal with the group shown in the key by test article and dose in mL/kg. Regression results of slope and 95% confidence interval in parenthesis. Dotted lines on the X and Y axes represent the LOD/LLOQ of the SNA assay (NT 80 12.3) and LLOQ of the RT-qPCR assay (931gc/mL), respectively.

Journal: NPJ Vaccines

Article Title: Passive transfer of human sera from chikungunya virus virus-like particle vaccine (Vimkunya) recipients fully protects non-human primates from viremia

doi: 10.1038/s41541-025-01360-1

Figure Lengend Snippet: A CHIKV RNAemia by RT-qPCR of plasma. Group log 10 gc/mL results with symbols representing the mean log 10 gc/mL of the NHP groups shown and error bars representing standard error of the mean. The validated CHIKV RT-qPCR assay LOD (dashed line) and LLOQ (dotted line) were 292 and 931 gc/mL, respectively. Results

Article Snippet: The CHIKV virus-like particle (VLP) vaccine (Vimkunya, previously PXVX0317, Bavarian Nordic) contains 40 μg of CHIKV VLP adjuvanted with aluminum hydroxide and is provided as a single-dose pre-filled syringe for intramuscular injection.

Techniques: Quantitative RT-PCR, Clinical Proteomics, Transformation Assay

Relationship between Day 0 SNA NT 80 and RNAemia as measured by RT-qPCR for CHIKV VLP immune sera- or IgG-treated NHPs with RNAemia ≥ LLOQ detected on at least 1 day (detectable viremia) or undetectable on all days (no viremia). Group 6 negative control animals are not shown.

Journal: NPJ Vaccines

Article Title: Passive transfer of human sera from chikungunya virus virus-like particle vaccine (Vimkunya) recipients fully protects non-human primates from viremia

doi: 10.1038/s41541-025-01360-1

Figure Lengend Snippet: Relationship between Day 0 SNA NT 80 and RNAemia as measured by RT-qPCR for CHIKV VLP immune sera- or IgG-treated NHPs with RNAemia ≥ LLOQ detected on at least 1 day (detectable viremia) or undetectable on all days (no viremia). Group 6 negative control animals are not shown.

Article Snippet: The CHIKV virus-like particle (VLP) vaccine (Vimkunya, previously PXVX0317, Bavarian Nordic) contains 40 μg of CHIKV VLP adjuvanted with aluminum hydroxide and is provided as a single-dose pre-filled syringe for intramuscular injection.

Techniques: Quantitative RT-PCR, Negative Control

The y-axis represents the probability of immunity/protection from CHIKV, the x-axis represents the Day 0 NT 80 , and each symbol is an individual NHP (Observed), with points jittered around 0.0 and 1.0 to view all animals. The solid curve represents the predicted probability of immunity/protection (Predicted), the shaded area represents the 95% confidence interval for the prediction (95% CI), the dotted black line represents the SNA assay LOD/LLOQ of 12.3, and dashed red lines represent the Day 0 SNA titers associated with 80% or 90% probabilities (shown by solid horizontal red lines at 0.8 and 0.9, respectively) of immunity/protection of 23.6 and 25.9, respectively. Day 0 SNA titers < LOD/LLOQ were imputed as LOD/LLOQ to be conservative.

Journal: NPJ Vaccines

Article Title: Passive transfer of human sera from chikungunya virus virus-like particle vaccine (Vimkunya) recipients fully protects non-human primates from viremia

doi: 10.1038/s41541-025-01360-1

Figure Lengend Snippet: The y-axis represents the probability of immunity/protection from CHIKV, the x-axis represents the Day 0 NT 80 , and each symbol is an individual NHP (Observed), with points jittered around 0.0 and 1.0 to view all animals. The solid curve represents the predicted probability of immunity/protection (Predicted), the shaded area represents the 95% confidence interval for the prediction (95% CI), the dotted black line represents the SNA assay LOD/LLOQ of 12.3, and dashed red lines represent the Day 0 SNA titers associated with 80% or 90% probabilities (shown by solid horizontal red lines at 0.8 and 0.9, respectively) of immunity/protection of 23.6 and 25.9, respectively. Day 0 SNA titers < LOD/LLOQ were imputed as LOD/LLOQ to be conservative.

Article Snippet: The CHIKV virus-like particle (VLP) vaccine (Vimkunya, previously PXVX0317, Bavarian Nordic) contains 40 μg of CHIKV VLP adjuvanted with aluminum hydroxide and is provided as a single-dose pre-filled syringe for intramuscular injection.

Techniques: